Molecular biomethods h andbook rapley ralph walker john m. Molecular Biomethods Handbook 2019-03-08

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WO2004020453A2

molecular biomethods h andbook rapley ralph walker john m

In spite of the attachment of other ligands to nanoparticles, there is no report of the use of a bifunctional peptide being used in conjunction with nanoparticles or other nanostructures for the generation of nano- scale conducting devices. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed. The reaction was exothermic; the heat generated in the reaction kept the solution warm for ˜15 min. Total reaction volume was brought up to 10 jaL with H2O. Genetically engineered phage could be used to present peptides as segments of their native surface proteins. Thus, the smallest diameter tube is encapsulated by a larger diameter tube, which in 35 turn, is encapsulated by another larger diameter nanotube.

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Molecular biomethods handbook in SearchWorks catalog

molecular biomethods h andbook rapley ralph walker john m

Figure 2 is a gel retardation assay. The bifunctional peptide will typically have at least two binding domains. For example, the peptide may be synthesized by solid state peptide synthesis where amino acids are sequentially added on a resin and then cleaved off the resin for purification see for example, Yokum et al. Preferred capture moieties are nucleic acids. The growth of the binding site unit is stopped by two pairs of capping primers on the two ends. Suitable shielding components will include but are not limited to short chain ethylene glycol oligomers, ethylene glycol methacrylate, sugars, crown ethers, and acrylamide, where the short chain ethylene glycol oligomers are preferred.

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Secret Bases • JN

molecular biomethods h andbook rapley ralph walker john m

These fragments may then be cloned into cloning and expression vectors that are suitable for the transformation of various microbial expression hosts. By linking the metal particles with organic semiconductors, it will be possible to develop 2-terminal switching devices, showing, for example, negative differential resistance e. A reducing agent was then added to reduce the metal salt to the free metal. Alternatively the capture moiety may be a biopolymer such as a nucleic acid matrix or a peptides or population of peptides having a specific structural conformation. Du Pont De Nemours And Company Priority date The priority date is an assumption and is not a legal conclusion. Nanometer-scale Electronic Device 35 The metallic or semiconductor nanoparticles of the invention, immobilized on a capture moiety matrix may be used in the construction of conductive heterojunctions and interconnects at the nano scale.

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Secret Bases • JN

molecular biomethods h andbook rapley ralph walker john m

Altered forms may be used for the purpose of the present invention. The sequences are shown below. Alexandra Aitken Selina Raguz and Michael Antoniou. Nature 395, 878-879 1998 ; A. Thus it is within the scope of the invention to provide functionalized nanoparticle comprising: a a nanoparticle coated with a monolayer comprising a capture 35 coating component; 13 b a bifunctional protein having a first binding domain and a second binding domain, the first and second binding domains each comprising a member of a binding pair; wherein the bifunctional protein is affixed to the nanoparticle of a 5 at the first binding domain. In general, the aspect ratio is between 10 and 2000.

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9781603273701

molecular biomethods h andbook rapley ralph walker john m

The reaction was exothermic; the heat generated in the reaction kept the solution warm for ~ 15 min. After incubation at room temperature for 10 min, the entire reaction mixture was loaded on 1% agarose gel. Preferred capture moieties are nucleic acids. Microbial expression systems and expression vectors containing regulatory sequences, such as promoters, that direct high level expression of foreign proteins are well known to those skilled in the art. A functionalized nanoparticle according to Claim 4 wherein the semiconductor comprising the nanoparticle is selected from the group consisting of cadmium selenide, cadmium sulfide, silver sulfide, cadmium sulfide, zinc sulfide, zinc selenide, lead sulfide, gallium arsenide, silicon, tin oxide, iron oxide, and indium phosphide. Each nucleic acid binding region will have a specific affinity for a 30 specific stretch of nucleic acids. The generation of random libraries of libraries of peptides is well known and may be accomplished by a variety of techniques including, bacterial display Kemp, D.

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Molecular biomethods handbook in SearchWorks catalog

molecular biomethods h andbook rapley ralph walker john m

Southern Blot Analysis Paolo A. The coenzyme A protected gold nanoparticles were prepared in a similar manner by substituting coenzyme A for tiopronin in the reaction. Science 281:2016-2018 1998 ; Mitchell et al. It is contemplated that a multiplicity of functionalized nanoparticles may be constructed each comprising a bifunctional peptide having a specific nucleic acid binding domain. Accordingly, a chimeric gene may comprise regulatory sequences and coding sequences that are derived from different sources, or regulatory sequences and coding sequences derived from the same source, but arranged in a manner different than that found in nature. Thus it is within the scope of the invention to provide functionalized nanoparticle comprising: a a nanoparticle coated with a monolayer comprising a capture coating component; b a bifunctional protein having a first binding domain and a second binding domain, the first and second binding domains each comprising a member of a binding pair; wherein the bifunctional protein is affixed to the nanoparticle of a at the first binding domain.

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US20040058457A1

molecular biomethods h andbook rapley ralph walker john m

A functionalized carbon nanotube comprising: a a carbon nanotube; and b a bifunctional protein having a first binding domain and a second binding domain, the first binding domain having affinity for a carbon nanotube and the second binding domain comprising a member of a binding pair; wherein the bifunctional protein is affixed to the carbon nanotube of a through the first binding domain. In the 24 metallic case, the immobilized nanoparticles are expected to be able to link nanometer scale electronic devices together permitting the fabrication of high density electronic circuits. Hydrophobic Interaction Chromatography Paul A. Yeast Artificial ChromosomesAngela Flannery and Rakesh Anand. The generation of random 30 libraries of libraries of peptides is well known and may be accomplished by a variety of techniques including, bacterial display Kemp, D. The ability to use biomolecules as templates in 15 many of these applications depends on how well one can achieve rational design based on specific binding between inorganic nanomaterials and biological molecules. Alternatively the capture moiety may be a biopolymer such as a nucleic acid matrix or a peptides or population of peptides having a specific structural conformation.

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Molecular Biomethods Handbook

molecular biomethods h andbook rapley ralph walker john m

The reaction was exothermic; the heat generated in the reaction kept the solution warm for ~ 15 min. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list. A similar method of preparing stabilized, water-soluble 15 nanoparticles of the metals gold, silver, platinum, palladium, cobalt and nickel is descried by Heath et al. Coated Nanoparticles The invention provides a nanoparticle coated with a monolayer that serves as a point of attachment for a bifunctional peptide. Enquist, Experiments with Gene Fusions, Cold Spring Harbor Laboratory, Cold Spring Harbor, N. A method according to Claim 18 wherein the functionalized nanoparticle comprises: a a nanoparticle coated with a monolayer comprising a capture coating component; b a bifunctional protein having a first binding domain and a second binding domain, the first binding domain comprising a member of a binding pair, the second binding domain comprising a nucleic acid binding amino acid sequence; wherein the bifunctional protein is affixed to the nanoparticle of a through the first binding domain.

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Secret Bases • JN

molecular biomethods h andbook rapley ralph walker john m

Clones were obtained containing between 1 to 23 Zif268 binding sites in tandem array. Conversely the shielding component forms the major component of the monolayer and comprises at least about 50% of the monolayer, where 60% to about 90% is preferred. The symbols and format used for nucleotide and amino acid sequence data comply with the rules set forth in 37 C. Microbial expression systems and expression vectors containing regulatory sequences, such as promoters, that direct high level expression 35 of foreign proteins are well known to those skilled in the art. The functionalized nanoparticles may be immobilized on matrices of biopolymers to form an electrical conducting network that will serve as the foundation for nano-circuitry. A nucleic acid nanoparticle complex comprising: a a nanoparticle coated with a monolayer comprising a capture coating component; b a bifunctional protein having a first binding domain and a second binding domain, the first binding domain comprising a member of a binding pair, the second binding domain comprising a nucleic acid binding amino acid sequence; wherein the bifunctional protein is affixed to the nanoparticle of a through the first binding domain and is affixed to a nucleic acid fragment at nucleic acid binding amino acid sequence. Radiolabeling of Peptides and Proteins Arvind C.

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Molecular biomethods handbook [electronic resource].

molecular biomethods h andbook rapley ralph walker john m

Protein motifs which recognize such sites include viral coat protein subunits Rossmann, M. Monoclonal Antibodies Christopher Dean and Helmout Modjtahedi. The capture component may be functionalized with various chemical groups that allow 20 for binding to a capture moiety. The nanoparticle is coated with a monolayer that is functionalized to recived a bi-functional protein. Annealing and ligation reactions were done according to the standard procedure Sambrook, J. These fragments may then be cloned into cloning and expression vectors that are suitable for the transformation of various microbial expression hosts. Accordingly in one embodiment the invention provides a functionalized nanoparticle comprising: a a nanoparticle coated with a monolayer comprising a 30 capture coating component; b a bifunctional protein having a first binding domain and a second binding domain, the first and second binding domains each comprising a member of a binding pair; wherein the bifunctional protein is affixed to the nanoparticle of a 35 at the first binding domain.

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